Malondialdehyde-modified HDL particles elicit a specific IgG response in abdominal aortic aneurysm

High Density Lipoprotein (HDL) plays a protective role in abdominal aortic aneurysm (AAA); however, recent findings suggest that oxidative modifications could lead to dysfunctional HDL AAA. This study aimed at testing the effect of oxidized on lesions and humoral immune responses mouse model AAA induced by elastase, evaluating whether antibodies against modified can be found patients. particles were with malondialdehyde (HDL-MDA) changes studied biochemical proteomics approaches. Experimental was mice elastase perfusion then treated HDL-MDA, or vehicle for 14 days. Aortic histomorphometric analysis. Levels anti-HDL-MDA IgG measured an in-house immunoassay model, human tissue-supernatants plasma samples from VIVA cohort. oxidation MDA confirmed enhanced susceptibility diene formation. Proteomics demonstrated presence adducts Lysine residues proteins, mainly ApoA-I. MDA-modification abrogated cultured endothelial cells as well dilation mice. Exposure HDL-MDA elicited response Anti-HDL-MDA also detected tissue-conditioned media patients, intraluminal thrombus. Higher levels patients compared controls. associated smoking independent predictors overall mortality Overall, MDA-oxidized trigger specific Besides, tissue suggesting its potential use surrogate stable biomarkers stress